Automated detection avoids variability associated with visual estimation. A. Mean ABR threshold shift for stem cell-transplanted and sham-injected mice in response to click and tone stimuli as estimated by visual inspection. The mean value for each animal was calculated from the data of ten independent observers. Comparisons of the two cohorts reveal similar results as for data analyzed by automated detection (see Figure 3). B. Examination of the data for individual observers shows that most observers did not detect a significant difference between stem cell-transplanted and sham-injected mice (NS) while some observers noted a significant difference in threshold shifts (S; p < 0.05). This variability in significance levels differed between click and tone stimuli.
Bogaerts et al. BMC Neuroscience 2009 10:104 doi:10.1186/1471-2202-10-104