FIZ1 can interact directly with CRX. Yeast (L40) were transformed with plasmids that express LexA-hybrid proteins (pHybLex/Zeo-bFIZ or pHybLex/Zeo-Laminin) combined with Gal4-AD-hybrids (pACT2 or pACT2-bCRX). LexA-Laminin and Gal4-AD were negative controls. Protein interactions were tested by A) growth on minus-His medium, and B) β-galactosidase activity by filter lift assay. C) Autoradiogram of GST pull-down assays. 35S-CRX protein (input) was prepared by in vitro transcription/translation and processed with glutathione-Sepharose-GST-bFIZ1 or glutathione-Sepharose-GST, using equal amounts of GST-bFIZ1 or GST protein.
Mali et al. BMC Molecular Biology 2008 9:87 doi:10.1186/1471-2199-9-87