Methodology article
Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi
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* Corresponding author: Rasmus JN Frandsen raf@life.ku.dk
1 Section of Genetics and Microbiology, Department of Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark
2 Department of Systems Biology, Technical University of Denmark, Søltofts Plads, Building 224, room 220, DK-2800 Lyngby, Denmark
3 Faculty of Agricultural Sciences, University of Aarhus, Blichers Allé 20, DK-8830 Tjele, Denmark
BMC Molecular Biology 2008, 9:70 doi:10.1186/1471-2199-9-70
Published: 1 August 2008Additional files
Additional File 1:
Table S1 – Oligonucleotides used in this study. The primer pairs used in the study.
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Additional File 2:
Vector sequences. The vector sequences in GenBank format.
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Additional File 3:
Protocols for USER Friendly cloning.
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