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Open Access Highly Accessed Research article

Quantification of mRNA in single cells and modelling of RT-qPCR induced noise

Martin Bengtsson12*, Martin Hemberg35, Patrik Rorsman1 and Anders Ståhlberg46*

Author Affiliations

1 Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, The Churchill Hospital, Oxford, OX3 7LJ, UK

2 Department of Clinical Sciences, Lund University, Clinical Research Centre, 205 02 Malmö, Sweden

3 Department of Bioengineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK

4 Stem Cell Center, Lund University, BMC B10, 221 84 Lund, Sweden

5 Department of Ophthalmology and Program in Neurobiology, Children's Hospital Boston, Harvard Medical School, 1 Blackfan Circle, Boston, MA 02115, USA

6 Department of Clinical Neuroscience and Rehabilitation, Institute of Neurosciences and Physiology, Sahlgrenska Academy at Göteborg University, Medicinaregatan 9A, 413 90 Göteborg, Sweden

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BMC Molecular Biology 2008, 9:63  doi:10.1186/1471-2199-9-63

Published: 17 July 2008

Additional files

Additional file 1:

containing Figures 1, 2, 3, 4 and Tables 1–3.

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Additional file 2:

containing a detailed description of the mathematical model.

Format: PDF Size: 120KB Download file

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