Quantification of mRNA in single cells and modelling of RT-qPCR induced noise

¹ Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, The Churchill Hospital, Oxford, OX3 7LJ, UK

² Department of Clinical Sciences, Lund University, Clinical Research Centre, 205 02 Malmö, Sweden

³ Department of Bioengineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK

⁴ Stem Cell Center, Lund University, BMC B10, 221 84 Lund, Sweden

⁵ Department of Ophthalmology and Program in Neurobiology, Children's Hospital Boston, Harvard Medical School, 1 Blackfan Circle, Boston, MA 02115, USA

⁶ Department of Clinical Neuroscience and Rehabilitation, Institute of Neurosciences and Physiology, Sahlgrenska Academy at Göteborg University, Medicinaregatan 9A, 413 90 Göteborg, Sweden