A siRNA directed against GFP specifically targets endogenous genes in human cells. (a) After transfection of the control siRNA directed against GFP, underrepresentation of the off-target genes CYLD and SOAT was detected by expression-microarrays 48 h post-transfection in HEK and HeLa cells. Normalization was performed as described in Additional file 5. (b) Verification of underrepresentation of CYLD and SOAT in HEK and HeLa cells by Real-Time PCR using the same mRNA as template. Expression of genes was normalized to the median of 2 housekeeping genes (PGK, DCTN2).
Tschuch et al. BMC Molecular Biology 2008 9:60 doi:10.1186/1471-2199-9-60