Table 5

Description of soybean genes for qRT-PCR

Namea

Soybase Accession Number

Arabidopsis homolog locusb

Arabidopsis locus description

Function

BLASTX score/E Value


ACT11

TC204137

AT3G12110

Actin11

Cytoskeletal structural protein

740/0

ACT2/7

TC204150

AT5G09810

Actin2/7

Cytoskeletal structural protein

739/0

G6PD

TC224599

AT5G40760

Glucose-6-phosphate dehydrogenase

Glucose metabolic process

903/0

ELF1B

TC203623

AT5G19510

Eukaryotic elongation factor 1-beta

Translational elongation

245/2e-65

UBC2

TC214734

AT2G02760

Ubiquitin-conjugating enzyme E2

Ubiquitin-dependent protein catabolic process

314/5e-86

ELF1A

TC203954

AT5G60390

Eukaryotic elongation factor 1-alpha

Translational elongation

469/e-132

TUB

TC203804

AT1G50010

Beta-tubulin

Structural constituent of cytoskeleton

847/0

TUA

AY907702

AT5G19780

Tubulin alpha-5

Structural constituent of cytoskeleton

803/0

CYP2

TC224926

AT2G21130

Cyclophilin

Protein folding

283/9e-77

UBQ10

TC203625

AT4G05320

Ubiquitin 10

Protein binding, protein modification process

734/0

GmBFT

EF532597

AT5G62040

Brother of FT and TFL1 protein

Phosphatidylethanola -mine binding

231/4e-61


aAll soybean sequences except TUA were ESTs based on the Arabidopsis proteins determined via BLASTX. bClosest Arabidopsis homolog identified using Tair BLAST [50]. AGI protein database was queried with soybean nucleotide sequences using BLASTX.

Jian et al. BMC Molecular Biology 2008 9:59   doi:10.1186/1471-2199-9-59

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