Table 1

Summary of statistics measuring stability of HKG expression

Gene

Mean Cta

StdDevb

Slopec

Interceptd

CV%e

Stability indexf


ACT11

18.63

0.74

0.03

20.97

3.57

0.11

CYP2

18.22

1.09

0.02

18.46

5.99

0.13

UBC2

21.91

1.17

0.03

21.91

5.33

0.16

ELF1B

23.50

1.21

0.05

24.01

5.16

0.24

ACT2/7

22.82

1.42

0.05

23.33

6.21

0.28

G6PD

22.91

1.68

0.04

22.45

7.34

0.31

UBQ10

22.85

1.53

0.06

22.14

6.68

0.43

TUB

21.77

1.74

0.09

22.80

7.99

0.72

TUA

21.47

1.42

0.12

22.86

6.63

0.80

ELF1A

19.25

1.43

0.12

20.58

7.45

0.90


aData based on analysis of raw Ct value. Genes were ordered, top to bottom, from those tending to show the highest stability to those showing the lowest, based on the stability index. bStdDev among all the tissue samples tested. cSlope of regression of gene means. dIntercepts were also given for the estimated regression lines. eCoefficient of variation (CV) (StdDev divided by mean multiplied by 100). fStability index was the product of CV and slope (multiplication of columns 4 and 6). Genes with the lowest stability index usually provide the best control.

Jian et al. BMC Molecular Biology 2008 9:59   doi:10.1186/1471-2199-9-59

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