Relative quantification of GmBFT expression using different HKGs as internal controls under different developmental stages and photoperiod conditions. Relative quantification of GmBFT expression was detected using two or three of the most stable HKGs or the most variable HKGs selected by geNorm as internal controls. The geometric average of ACT2/7, TUA and ELF1A, UBQ10 and G6PD were used as internal controls for developmental stage (A), the geometric average of ACT11 and ELF1B, UBQ10 and G6PD were used as internal controls for photoperiod treatment (B). a, SD 1-day leaves; b, SD 25-day leaves; c, SD 1-day shoot tips; d, SD 25- day shoot tips; e, LD 25-day leaves; f, LD 25-day shoot tips.
Jian et al. BMC Molecular Biology 2008 9:59 doi:10.1186/1471-2199-9-59