HER911 cells express endogenous clock genes on the mRNA level. cDNA of HER911 cells was obtained by reverse transcription of total RNA. hPer1, hPer2, hCry1, hCry2, hBmal1 and hClock cDNAs were amplified by PCR (lanes c). As positive control and to distinguish between true cDNA products (black arrowheads) and products from contaminating genomic DNA (gray arrowheads), a parallel reaction was run using human genomic DNA as template (lanes g). A negative control was done with water (lanes n).
Langmesser et al. BMC Molecular Biology 2008 9:41 doi:10.1186/1471-2199-9-41