Mapping of PER2, CRY1 and CRY2 binding sites in BMAL1. HER911 cells were co-transfected with pFR-luc and μg pCMV-lacZ together with the indicated expresssion plasmids. For each experiment (n = 5–7), values obtained for cells transfected with the luciferase reporter alone were set to 1. Significant luciferase reporter activation by pSCT1-Per2/Cry1/Cry2-VP16 as compared to the respective pSCT1-Gal4-Bmal1 construct alone was determined by student's t-test (* p < 0.05, ** p < 0.01, *** p < 0.001).
Langmesser et al. BMC Molecular Biology 2008 9:41 doi:10.1186/1471-2199-9-41