PER2, CRY1 and CRY2 all interact with BMAL1. (A) and (C) HER911 cells were co-transfected with 3 μg pFR-luc, 0.1 μg pCMV-lacZ and the indicated expresssion plasmids. For each experiment (n = 6), values obtained for cells transfected with the luciferase reporter alone were set to 1. *** p < 0.001 as determined by student's t-test compared to all other columns. (B), (D) and (E) HER911 cells were co-transfected with pSCT1-Bmal1-GFP and pSCT1-Per2 (B), pSTC-TK-Cry1 (D) or pSTC-TK-Cry2 (E). Total cell extracts (left panels) or immunoprecipitates using an anti-GFP antibody (right panels) were subjected to Western blotting using antibodies against PER2, CRY1 and CRY2, respectively. Blots are representative results from one experiment, all co-immunoprecipitations were repeated twice. WB Western Blot, IP immunoprecipitation.
Langmesser et al. BMC Molecular Biology 2008 9:41 doi:10.1186/1471-2199-9-41