Table 2

Methylation of CpG sites in the TLR2 promoter is significantly different between non-CF and CF epithelial cellsa

CpG#

Sequences

Methylated

Unmethylated

p values


#1–3

non-CF

43

46

83

CF

41

17

106

7.277 × 10-5***

#4–5

non-CF

43

18

68

CF

41

22

60

0.4698

#6–8

non-CF

43

8

121

CF

41

1

122

0.0362*

#9–10

non-CF

43

3

83

CF

41

1

81

0.6209

#11–12

non-CF

43

6

80

CF

41

1

81

0.1179

#13–15

non-CF

43

20

109

CF

41

8

115

0.0273*

#16–17

non-CF

43

12

74

CF

41

3

79

0.0282*

#18–20

non-CF

43

22

107

CF

41

4

119

0.0003***


aCpG methylation was scored at specific sites (CpG#1-3, 4–5, 6–8, 9–10, 1–12, 13–15, 16–17 and 18–20) in the TLR2 promoter sequence from non-CF (16HBE14o-, n = 23; HeLa, n = 10; A549, n = 10) and CF (CFBE41o-, n = 21; CFTE41o-, n = 10; CFPAC-1, n = 10) epithelial cells. The number of methylated and unmethylated sites was scored and a Fischer's exact test was used to assign p values (*, p < 0.05; ***, p < 0.001).

Furuta et al. BMC Molecular Biology 2008 9:39   doi:10.1186/1471-2199-9-39

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