Inter-assay variance of Ct improvement in EMBs using T-PreAmp. Ct improvement achieved by T-PreAmp from 4 different EMBs (illustrated in 4 different colors) for each gene assay. The individual Ct values of CDKN1B and HPRT-CCM are not shown, since they were determined in all 38 EMBs.
Noutsias et al. BMC Molecular Biology 2008 9:3 doi:10.1186/1471-2199-9-3