T-PreAmp procedure. Principle of the T-PreAmp technique: After standard reverse transcription, cDNA was preamplified (14 cycles) using a pool of all investigated self-designed gene assays (primers) and ABI inventoried Taqman® gene expression assays (mixture of primers and fluorescence probes). The preamplified cDNA products were then diluted 1:20 and subjected to real-time PCR analyses.
Noutsias et al. BMC Molecular Biology 2008 9:3 doi:10.1186/1471-2199-9-3