Methodology article
Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies
- † Equal contributors
1 Department of Cardiology and Pneumonology, Charité – Universitätsmedizin Berlin, Campus Benjamin Franklin, 12200 Berlin, Germany
2 Institute of Medical Immunology, Charité – Universitätsmedizin Berlin, Campus Mitte, 10117 Berlin, Germany
3 Department of Pathology, Charité – Universitätsmedizin Berlin, Campus Benjamin Franklin, 12200 Berlin, Germany
4 Deutsches Herzzentrum Berlin, 13353 Berlin, Germany
BMC Molecular Biology 2008, 9:3 doi:10.1186/1471-2199-9-3
Published: 14 January 2008Additional files
Additional file 1:
T-PreAmp real-time RT-PCR gene expression results of all investigated genes in EMBs from DCM and DCMi patients related to HPRT-CCM. Gene expression (E; normalized to HPRT-CCM) T-PreAmp real-time RT-PCR results in EMBs from DCM patients compared with EMBs from patients with immunohistologically confirmed DCMi (>7 CD3+ infiltrates/mm2). The values are given as means ± SD, followed by the respective p values in significantly different comparisons. Non-significant comparisons are denoted as n.s.. All investigated genes were quantified out of one single T-PreAmp reaction per EMB.
Format: DOC Size: 140KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional file 2:
T-PreAmp real-time RT-PCR gene expression results of all investigated genes in EMBs from DCM and DCMi patients related to CDKN1B. Gene expression (E; normalized to CDKN1B) T-PreAmp real-time RT-PCR results in EMBs from DCM patients compared with EMBs from patients with immunohistologically confirmed DCMi (>7 CD3+ infiltrates/mm2). The values are given as means ± SD, followed by the respective p values in significantly different comparisons. Non-significant comparisons are denoted as n.s.. All investigated genes were quantified out of one single T-PreAmp reaction per EMB.
Format: DOC Size: 146KB Download file
This file can be viewed with: Microsoft Word Viewer
Additional file 3:
List of abbreviations. The table enlists abbreviations commonly used in this manuscript.
Format: DOC Size: 46KB Download file
This file can be viewed with: Microsoft Word Viewer
