Open Access Highly Accessed Methodology article

Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies

Michel Noutsias1*, Maria Rohde1, Andrea Block1, Katrin Klippert2, Olga Lettau1, Katja Blunert13, Michael Hummel3, Uwe Kühl1, Hans Lehmkuhl4, Roland Hetzer4, Ursula Rauch1, Wolfgang Poller1, Matthias Pauschinger1, Heinz P Schultheiss1, Hans D Volk2 and Katja Kotsch2

Author Affiliations

1 Department of Cardiology and Pneumonology, Charité – Universitätsmedizin Berlin, Campus Benjamin Franklin, 12200 Berlin, Germany

2 Institute of Medical Immunology, Charité – Universitätsmedizin Berlin, Campus Mitte, 10117 Berlin, Germany

3 Department of Pathology, Charité – Universitätsmedizin Berlin, Campus Benjamin Franklin, 12200 Berlin, Germany

4 Deutsches Herzzentrum Berlin, 13353 Berlin, Germany

For all author emails, please log on.

BMC Molecular Biology 2008, 9:3  doi:10.1186/1471-2199-9-3

Published: 14 January 2008

Additional files

Additional file 1:

T-PreAmp real-time RT-PCR gene expression results of all investigated genes in EMBs from DCM and DCMi patients related to HPRT-CCM. Gene expression (E; normalized to HPRT-CCM) T-PreAmp real-time RT-PCR results in EMBs from DCM patients compared with EMBs from patients with immunohistologically confirmed DCMi (>7 CD3+ infiltrates/mm2). The values are given as means ± SD, followed by the respective p values in significantly different comparisons. Non-significant comparisons are denoted as n.s.. All investigated genes were quantified out of one single T-PreAmp reaction per EMB.

Format: DOC Size: 140KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 2:

T-PreAmp real-time RT-PCR gene expression results of all investigated genes in EMBs from DCM and DCMi patients related to CDKN1B. Gene expression (E; normalized to CDKN1B) T-PreAmp real-time RT-PCR results in EMBs from DCM patients compared with EMBs from patients with immunohistologically confirmed DCMi (>7 CD3+ infiltrates/mm2). The values are given as means ± SD, followed by the respective p values in significantly different comparisons. Non-significant comparisons are denoted as n.s.. All investigated genes were quantified out of one single T-PreAmp reaction per EMB.

Format: DOC Size: 146KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 3:

List of abbreviations. The table enlists abbreviations commonly used in this manuscript.

Format: DOC Size: 46KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data