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Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa

Biliana Lesic123 and Laurence G Rahme123*

Author Affiliations

1 Department of Surgery, Harvard Medical School and Massachusetts General Hospital, Boston, MA 02114, USA

2 Shriners' Research Institute, Boston, MA 02114, USA

3 Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA

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BMC Molecular Biology 2008, 9:20  doi:10.1186/1471-2199-9-20

Published: 4 February 2008

Additional files

Additional File 1:

Schematic map of pUCP18-RedS. The schematic representation of pUC18-RedS (Genbank EU073163) includes the Red operon (gam, bet, exo) – araC fragment, sacB encoding the levansucrase, and bla encoding the resistance to ampicillin.

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Additional File 2:

Primers used in this study. The table lists all primers used in the various mutagenesis steps described in this paper.

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Open Data