BMC Molecular Biology

official impact factor 3.19

Open Access Research article

Specificity of DNA-binding by the FAX-1 and NHR-67 nuclear receptors of Caenorhabditis elegans is partially mediated via a subclass-specific P-box residue

Stephen D DeMeo1,2, Rebecca M Lombel1,3, Melissa Cronin1, Eric L Smith1,4, Danielle R Snowflack1,5, Kristy Reinert1,6, Sheila Clever1 and Bruce Wightman1*

Author Affiliations

1 Biology Department, Muhlenberg College, Allentown, PA 18104, USA

2 Current address : Philadelphia College of Osteopathic Medicine, Philadelphia, PA 19131, USA

3 Current address : Department of Pediatrics, University of Michigan Health System, Ann Arbor, MI 48109, USA

4 Current address : Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, NY 10029, USA

5 Current address : Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA

6 Current address : School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA

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BMC Molecular Biology 2008, 9:2 doi:10.1186/1471-2199-9-2

Published: 7 January 2008

Additional files

Additional File 1:

Paired oligonucleotide sequences used for generating binding sites for EMSA and one-hybrid experiments. Complete oligonucleotide sequences evaluated in this study.

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Open Data

Additional File 2:

Supplementary methods. Details of plasmid construction and experimental conditions for EMSA.

Format: PDF Size: 24KB Download file

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Open Data