Table 2 |
||||||
|
Real-time quantitative PCR characteristics |
||||||
|
Gene |
Accesion No. |
Forward primer |
Reverse primer |
Annealing °C |
MgCl2 mM |
Amplicon size (bp) |
|
|
||||||
|
HMBS |
NM_000190 |
ggcaatgcggctgcaa |
gggtacccacgcgaatcac |
56 |
2.5 |
64 |
|
TBP |
NM_003194 |
tgcacaggagccaagagtgaa |
cacatcacagctccccacca |
56 |
2.5 |
132 |
|
B2M |
NM_004048 |
atgagtatgcctgccgtgtga |
ggcatcttcaaacctccatg |
56 |
2.5 |
101 |
|
ACTB |
NM_001101 |
ggcatcctcaccctgaagta |
ggggtgttgaaggtctcaaa |
56 |
2.5 |
203 |
|
HPRT1 |
NM_000194 |
tgctcgagatgtgatgaagg |
tcccctgttgactggtcatt |
56 |
2.5 |
192 |
|
RPL13A |
NM_012423 |
aaaaagcggatggtggttc |
cttccggtagtggatcttgg |
56 |
4.5 |
168 |
|
SDHA |
NM_004168 |
tggacctggttgtctttggt |
agtcgcagttccgatgttct |
56 |
3.5 |
166 |
|
UBC |
NM_021009 |
atcgctgtgatcgtcacttg |
tccagcaaagatcagcctct |
64 |
3.5 |
164 |
|
18S |
NM_022551 |
atccctgaaaagttccagca |
ccctcttggtgaggtcaatg |
56 |
2.5 |
186 |
|
GAPDH |
NM_002046 |
gagccacatcgctcagacac |
catgtagttgaggtcaatgaagg |
60 |
3.5 |
150 |
|
|
||||||
|
Pombo-Suarez et al. BMC Molecular Biology 2008 9:17 doi:10.1186/1471-2199-9-17 |
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