Table 1

Plasmids and yeast strains

Genotpye

Origin


Plasmid

pGK25

CEN ARS URA3 GFP

[24]

pGK26

CEN ARS URA3 SAC1 5' UTR (-500/-1)-GFP

[24]

pAK29

CEN ARS URA3 SAC1 5' UTR (-242/-1)-GFP

This study

pAK30

CEN ARS URA3 SAC1 5' UTR (-170/-1)-GFP

This study

pAK34

CEN ARS URA3 SAC1 5' UTR (-500/-150)-GFP

This study

pAK36

CEN ARS URA3 SAC1 5' UTR (-125/-1)-GFP

This study

pAK38

CEN ARS URA3 SAC1 5' UTR (-83/-1)-GFP

This study

PAK40

CEN ARS URA3 SAC1 5' UTR (-114/-1)-GFP

This study

pAK42

CEN ARS URA3 SAC1 5' UTR (-100/-1)-GFP

This study

pAK47

CEN ARS URA3 SAC1 5' UTR Δ(-100/-84)-GFP

This study

pAK48

CEN ARS URA3 SAC1 5' UTR Δ(-83/-70)-GFP

This study

pAK49

CEN ARS URA3 SAC1 5' UTR Δ(-91/-84)-GFP

This study

pAK50

CEN ARS URA3 SAC1 5' UTR Δ(-100/-92)-GFP

This study

Strain

ATY201

MATαtrp1-delta901 leu2-3,112 his3-delta200 ura3-52 lys2-801 suc2-delta9 can1::hisG

[36]

STY39

MATa trp1-delta901 leu2-3,112 his3-delta200 ura3-52 lys2-801 suc2-delta9 can1::hisG sac1::TRP

[8]

STY40

MATa leu2-3, 112 ura3-52 his3-delta200 trp1-delta901 lys2-801 suc2-delta9 sac1::TRP1 stt4::HIS3 pSTT4-4 (LEU2 CEN6 stt4-4)

[8]

STY47

Pik1::ADE2-1 sac1::TRP YEplac181::pik1-12

[8]

PMY434

MATαtrp1-delta901 leu2-3,112 his3-delta200 ura3-52 lys2-801 suc2-delta9 can1::hisG opi1::HIS3

This study

PMY435

MATa trp1-delta901 leu2-3,112 his3-delta200 ura3-52 lys2-801 suc2-delta9 can1::hisG sac1::TRP opi1::HIS3

This study


Knödler et al. BMC Molecular Biology 2008 9:16   doi:10.1186/1471-2199-9-16

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