Figure 12.

Wnt3a stimulation of STF mediated by Fzd2 and Ror2 is inhibited by GSK3. H441 cells were transfected with pSV-β-gal (Promega, WI), STF, Fzd2 and Ror2WT plus Xgsk3 expression construct, and then treated with L-CM or Wnt3aCM. Equal amounts of DNA and conditioned media were used in each assay. Luciferase values were normalized to beta-galactosidase for transfection efficiency. Values of the assay transfected with control vector and treated with L-CM alone (empty bars) were adjusted to unity to normalize all other experimental values in the same group. * indicates p < 0.05.