Additional file 1:

Immunoprecipitation control and RT-PCR. As an internal control, IP and RT-PCR reactions were performed using a non-related antibody, anti-TcRpi. The same conditions and amplification cycles were used. A) Western blot employing anti-TcRpi in immunoprecipitated samples. The position of the bands corresponding to each protein and to the heavy chain (hc) of antibodies is indicated with an arrow on the left side of the panel. Molecular mass protein standards Dalton Mark VII-L™ are indicated on the right side. B) PCR amplification using specific sets of primers to confirm the absence of some identified transcripts for TcUBP1 and TcRBP3. PS, preimmune serum; TcRpi, T. cruzi Ribose 5-phosphate isomerase Type B.

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Additional file 2:

List of database targets harboring UBP1m. Sequences obtained from dbEST bearing UBP1m were filtered using the annotation file provided by TIGR and manually classified into functional categories. N, number of sequences found.

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Additional file 3:

List of database targets bearing RBP3m. Sequences obtained from dbEST bearing RBP3m were filtered using the annotation file provided by TIGR and manually classified into functional categories. N, number of sequences found.

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Additional file 4:

Several transcripts have different motifs for either TcUBP1 or TcRBP3. This table contains a list of mRNA target hits bearing the highest four UBP1- and RBP3-motifs.

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Additional file 5:

PCR primers. This table contains a list of oligonucleotides used in this work for RT-PCR.

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Additional file 6:

Primers for motifs and deletions. This table contains a list of oligonucleotides used in this work for construction of motifs and deletions.

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