|
Housekeeping gene expression following vehicle/hormone/toxicant treatment from 24–96 hpf, as measured by QPCR |
||||||||
| Ct values +/- S.E.M. |
||||||||
|
|
||||||||
| GENE |
CONTROL |
ETOH |
DMSO |
0.1 μM E2 |
1 μM T |
10 μM ICI |
10 nM BNF |
1 nM TCDD |
|
|
||||||||
| bactin1 |
15.1 ± 0.07 |
14.9 ± 0.01 |
15.2 ± 0.12 |
15.0 ± 0.03 |
14.7 ± 0.08 |
15.1 ± 0.05 |
15.5 ± 0.19 |
15.1 ± 0.02 |
| tuba1 |
19.9 ± 0.11 |
19.5 ± 0.08 |
19.6 ± 0.08 |
20.0 ± 0.13 |
19.3 ± 0.08 |
19.8 ± 0.04 |
19.7 ± 0.05 |
19.5 ± 0.08 |
| gapdh |
16.8 ± 0.12 |
16.1 ± 0.20* |
16.0 ± 0.10* |
15.9 ± 0.02 |
15.9 ± 0.07 |
15.8 ± 0.03 |
15.9 ± 0.11 |
16.1 ± 0.06 |
| g6pd |
21.4 ± 0.11 |
20.7 ± 0.04* |
20.8 ± 0.03 |
21.5 ± 0.06 |
20.8 ± 0.01 |
20.8 ± 0.03 |
21.4 ± 0.16 |
21.1 ± 0.07 |
| tbp |
20.4 ± 0.53 |
21.6 ± 0.14 |
20.6 ± 0.31 |
21.4 ± 0.05 |
21.9 ± 0.15 |
21.9 ± 0.06 |
22.1 ± 0.13 |
22.3 ± 0.25 |
| b2m |
23.8 ± 0.16 |
23.0 ± 0.13 |
22.9 ± 0.23 |
23.6 ± 0.10 |
24.3 ± 0.43 |
24.0 ± 0.44 |
24.5 ± 0.30 |
24.2 ± 0.16 |
| elfa |
14.6 ± 0.12 |
14.2 ± 0.01* |
14.2 ± 0.01* |
14.2 ± 0.05 |
14.4 ± 0.12 |
14.3 ± 0.05 |
14.4 ± 0.07 |
14.3 ± 0.03 |
| 18s |
16.2 ± 0.02 |
16.3 ± 0.08 |
16.8 ± 0.18* |
16.3 ± 0.03 |
16.3 ± 0.03 |
16.3 ± 0.15 |
16.3 ± 0.02 |
16.9 ± 0.09 |
|
Values represent Ct values (mean +/- SEM) from 3 biological replicates (50 embryos/larvae per pool, 3 pools per treatment group). For details, see Methods and Results. Asterisks (*) indicate significant difference between vehicle [dimethyl sulfoxide (DMSO), ethanol (EtOH)] and control (control = untreated; p < .05). Boldface indicates significant difference between treatment [17β-estradiol (E2), testosterone (T), ICI 182,780 (ICI), β-napthaflavone (BNF), tetrachlodibenzo-p-dioxin (TCDD)] and DMSO (p < .05). | ||||||||
McCurley and Callard BMC Molecular Biology 2008 9:102 doi:10.1186/1471-2199-9-102 |
||||||||