Table 1

Genes selected for expression analysis
Gene Name	Gene Symbol	Cellular Function	Primer Sequence (5'-3')	Reference	Accession #

bactin1	bactin1	Cytoskeleton	F) CGAGCAGGAGATGGGAACC	14	AF057040
			R) CAACGGAAACGCTCATTGC

tubulin, alpha 1	tuba1	Cytoskeleton	F) CCTGCTGGGAACTGTATTGT	*	AF029250
			R) TCAATGAGTTCCTTGCCAAT

glyceraldehyde-3-phosphate dehydrogenase	gapdh	Glycolysis enzyme	F) GTGGAGTCTACTGGTGTCTTC	15	BC083506
			R) GTGCAGGAGGCATTGCTTACA

glucose-6-phosphate dehydrogenase	g6pd	Glycolysis enzyme	F) GTCCCGAAAGGCTCCACTC	9	BM182602
			R) CCTCCGCTTTCCTCTC

TATA-box-binding protein	tbp	Transcription	F) CGGTGGATCCTGCGAATTA	*	NM_200096
			R) TGACAGGTTATGAAGCAAAACAACA

beta-2-microglobulin	b2m	Major histocompatibility complex	F) GCCTTCACCCCAGAGAAAGG	*	BC062841
			R) GCGGTTGGGATTTACATGTTG

elongation factor 1-alpha	elfa	Translation	F) CTTCTCAGGCTGACTGTGC	16	AY422992
			R) CCGCTAGCATTACCCTCC

18s ribosomal RNA	18s	Ribosome subunit	F) TCGCTAGTTGGCATCGTTTATG	17	BX296557
			R) CGGAGGTTCGAAGACGATCA

cytochrome P450, family 19, subfamily A, polypeptide 1b	cyp19a1b	Steroid biosynthesis	F) AAAGAGTTACTAATAAAGATCCACCGGTAT	13	AF226619
			R) TCCACAAGCTTTCCCATTTCA

cytochrome P450, family 1, subfamily A	cyp1a	Xenobiotic metabolism	F) GCATTACGATACGTTCGATAAGGAC	18	NM_131879
			R) GCTCCGAATAGGTCATTGACGAT

The first 8 were candidate housekeeping genes. cyp19a1b and cyp1a, targets of ER and AhR mediated signal transduction, respectively, were used to tests effects of normalization on apparent gene expression. Asterisks (*) indicate primers designed in this study.
McCurley and Callard BMC Molecular Biology 2008 9:102 doi:10.1186/1471-2199-9-102