Housekeeping gene expression during development, as measured by QPCR. Embryos were collected at timed intervals (2, 6, 12, 24, 48, 72, 96, and 120 hpf) and pooled (50 embryos/pool, 3 pools per time point) for analysis. Ct values represent mean +/- SEM from three biological replicates. Note that the y-axis differs in the three panels to show gene groups based on Ct range during development: (top to bottom) highly expressed genes (bactin1, elfa, 18s), genes with highly variable expression (tuba1, gapdh), genes with moderate expression (tbp, b2m, g6pd). All genes showed significant differences across developmental stages by one-way ANOVA p < .05.
McCurley and Callard BMC Molecular Biology 2008 9:102 doi:10.1186/1471-2199-9-102