Additional File 1.

Gene repair at 8 hours following ssODN electroporation. Cells were treated either with or without 1 mM thymidine for 24 hours prior to the electroporation of 1 μM EGFP3S/47NT and a subset of the samples was allowed to recover in the presence of 2 mM thymidine. Gene repair was assayed by flow cytometry at 8 hours following the introduction of the ssODN and correction is given as the percent of GFP(+)/PI(-) [fluorescing, live] cells out of the 50,000 cells analyzed. The data shows that the pre-treatment with 1 mM thymidine resulted in a greater than a 5 fold increase in gene repair (0.12% vs. 0.66%), as compared to the non-treated sample. The addition of 2 mM thymidine in the recovery led to a 3 fold decrease in repair frequency of the pre-treated sample (0.21% vs. 0.66%) but did not alter the correction levels of the no pre-treated sample (0.11% vs. 0.12%).

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Engstrom et al. BMC Molecular Biology 2007 8:9   doi:10.1186/1471-2199-8-9