Figure 1.

A 24 hour pre-treatment with thymidine enhances gene repair levels. Various concentrations of thymidine were added to a growing culture of DLD-1 cells 24 hours prior to the introduction of 1 μM oligonucleotide (EGFP3S/47NT). Cells were allowed to recover for 48 hours after electroporation and were harvested for FACS analysis. Correction efficiency is reported as the percent of live green fluorescent cells in the total cell population (50,000 per experiment); mean and standard deviations were calculated based on values obtained from 3–4 samples per treatment. T-tests were performed comparing the 1 mM and 8 mM thymidine pre-treatment to the 0 mM sample (***p < 5 × 10^-5).