Heterodimer formation between DMRT1 and DMRT3. Gel mobility shifts using labeled DMRT1 consensus probe DNA and indicated in vitro translated proteins. Positions of monomeric, and homo- and heterodimeric complexes with DNA are indicated (maroon triangles indicate DMRT1 and gold triangles indicate DMRT3). DMRT3 protein is tagged with a myc epitope recognized by 9E10 monoclonal antibody. As shown in first two lanes, 9E10 does not affect complex formed by DMRT1, and anti-DMRT1 antibody does not affect complex formed by tagged DMRT3.
Murphy et al. BMC Molecular Biology 2007 8:58 doi:10.1186/1471-2199-8-58