Table 2

Characteristics of gene-specific real-time PCR assays

Gene symbol

Gene name

Accession No.

Primer/Probe Sequence [5'→3']

Amplicon Size [bp]

Detection Dye/Probe

PCR Efficiencyd


ACTB

Actin, beta

NM_001101

Forward: agcctcgcctttgccga

Reverse: ctggtgcctggggcg

Probe: F-ccgccgcccgtccacacccgccT-P

174

TMa

1.89

ALAS1

5-Aminolevulinate delta-, synthase 1

NM_000688

LightCycler-h-ALAS

Housekeeping Gene Set

Roche (Cat. No. 03 302 504 001)

127

Hybb

1.97

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

NM_002046

Forward: gaaggtgaaggtcggagtc

Reverse: gaagatggtgatgggatttc

Probe: F-caagcttcccgttctcagccT-P

226

TM

1.99

HMBS

Hydroxymethyl-bilane synthase Alias: Porphobilinogen deaminase (PBGD)

NM_000190

LightCycler-h-PBGD

Housekeeping Gene Set

Roche, Cat. No. 03 146 073 001

150

Hyb

1.98

HPRT1

Hypoxanthine phosphoribosyl transferase 1

NM_000194

LightCycler-h-HPRT

Housekeeping Gene Set

Roche (Cat. No. 03 261 891 001)

181

Hyb

2.00

PPIA

Peptidylprolyl isomerase A

Alias: Cyclophilin A

NM_021130

Hs_PPIA_1_SG QuantiTect Primer Assay

Qiagen (Cat. No. QT00052311)

121

SGIc

1.84

RPLPO

Ribosomal protein, large, PO

NM_001002

Hs_RPLPO_1_SG QuantiTect Primer Assay

Qiagen (Cat. No. QT00075012)

79

SGI

1.92

SDHA

Succinate dehydrogenase complex,

subunit A, flavoprotein (Fp)

NM_004168

Forward: cactggaggaagcacaccc

Reverse: ccttcccagtgccaacgtccacaat

Probe: F-ccttcccagtgccaacgtccacaaT-P

78

TM

1.92

TBP

TATA box binding protein

NM_003194

Forward: ttcggagagttctgggattgta

Reverse: tggactgttcttcactcttggc

Probe: F-ccgtggttcgtggctctcttatcctcaT-P

227

TM

1.88

TUBB

Tubulin, beta

NM_178014

Hs_TUBB_1_SG QuantiTect Primer Assay

Qiagen (Cat. No. QT00089775)

120

SGI

1.98

ADAM9

ADAM metallopeptidase domain 9,

(meltrin gamma)

NM_003816

Forward: ggtgacagatttggcaattgtg

Reverse: ttgtgccttcgttaaccatcc

Donor probe:

acgcctagtcgaggcaccaaatgttg-6Fl

Acceptor probe:

Cy5.5-gtgtggatttccagctaggatcagatgttcc-P

226

Hyb

1.95


aTM = TaqMan probes are 3'-end F, FAM (6-carboxy-fluorescein) and near 5'-end T, TAMRA (6-carboxy-tetramethyl-rhodamine) labelled and P, phosphorylated

bHyb = Hybridization probes, two different labelled probes are included in the kit (Roche); for target gene see text

cSGI = SYBR® Green I fluorescent dye

dPCR efficiencies were calculated according to Rasmussen [2].

Jung et al. BMC Molecular Biology 2007 8:47   doi:10.1186/1471-2199-8-47

Open Data