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Characteristics of gene-specific real-time PCR assays |
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| Gene symbol |
Gene name |
Accession No. |
Primer/Probe Sequence [5'→3'] |
Amplicon Size [bp] |
Detection Dye/Probe |
PCR Efficiencyd |
|
|
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| ACTB |
Actin, beta |
NM_001101 |
Forward: agcctcgcctttgccga Reverse: ctggtgcctggggcg Probe: F-ccgccgcccgtccacacccgccT-P |
174 |
TMa |
1.89 |
| ALAS1 |
5-Aminolevulinate delta-, synthase 1 |
NM_000688 |
LightCycler-h-ALAS Housekeeping Gene Set Roche (Cat. No. 03 302 504 001) |
127 |
Hybb |
1.97 |
| GAPDH |
Glyceraldehyde-3-phosphate dehydrogenase |
NM_002046 |
Forward: gaaggtgaaggtcggagtc Reverse: gaagatggtgatgggatttc Probe: F-caagcttcccgttctcagccT-P |
226 |
TM |
1.99 |
| HMBS |
Hydroxymethyl-bilane synthase Alias: Porphobilinogen deaminase (PBGD) |
NM_000190 |
LightCycler-h-PBGD Housekeeping Gene Set Roche, Cat. No. 03 146 073 001 |
150 |
Hyb |
1.98 |
| HPRT1 |
Hypoxanthine phosphoribosyl transferase 1 |
NM_000194 |
LightCycler-h-HPRT Housekeeping Gene Set Roche (Cat. No. 03 261 891 001) |
181 |
Hyb |
2.00 |
| PPIA |
Peptidylprolyl isomerase A Alias: Cyclophilin A |
NM_021130 |
Hs_PPIA_1_SG QuantiTect Primer Assay Qiagen (Cat. No. QT00052311) |
121 |
SGIc |
1.84 |
| RPLPO |
Ribosomal protein, large, PO |
NM_001002 |
Hs_RPLPO_1_SG QuantiTect Primer Assay Qiagen (Cat. No. QT00075012) |
79 |
SGI |
1.92 |
| SDHA |
Succinate dehydrogenase complex, subunit A, flavoprotein (Fp) |
NM_004168 |
Forward: cactggaggaagcacaccc Reverse: ccttcccagtgccaacgtccacaat Probe: F-ccttcccagtgccaacgtccacaaT-P |
78 |
TM |
1.92 |
| TBP |
TATA box binding protein |
NM_003194 |
Forward: ttcggagagttctgggattgta Reverse: tggactgttcttcactcttggc Probe: F-ccgtggttcgtggctctcttatcctcaT-P |
227 |
TM |
1.88 |
| TUBB |
Tubulin, beta |
NM_178014 |
Hs_TUBB_1_SG QuantiTect Primer Assay Qiagen (Cat. No. QT00089775) |
120 |
SGI |
1.98 |
| ADAM9 |
ADAM metallopeptidase domain 9, (meltrin gamma) |
NM_003816 |
Forward: ggtgacagatttggcaattgtg Reverse: ttgtgccttcgttaaccatcc Donor probe: acgcctagtcgaggcaccaaatgttg-6Fl Acceptor probe: Cy5.5-gtgtggatttccagctaggatcagatgttcc-P |
226 |
Hyb |
1.95 |
|
aTM = TaqMan probes are 3'-end F, FAM (6-carboxy-fluorescein) and near 5'-end T, TAMRA (6-carboxy-tetramethyl-rhodamine) labelled and P, phosphorylated bHyb = Hybridization probes, two different labelled probes are included in the kit (Roche); for target gene see text cSGI = SYBR® Green I fluorescent dye dPCR efficiencies were calculated according to Rasmussen [2]. | ||||||
Jung et al. BMC Molecular Biology 2007 8:47 doi:10.1186/1471-2199-8-47 |
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