Dynamic resolution of functionally related gene sets in response to acute heat stress
1 Developmental and Molecular Pathways, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA
2 Diabetes & Metabolism Disease Area, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA
BMC Molecular Biology 2007, 8:46 doi:10.1186/1471-2199-8-46Published: 5 June 2007
Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by microarray in a temporal fashion to characterize the dynamic relationship of signaling pathways.
Bioinformatics analyses exposed coordination of functionally-related gene sets, depicting mechanism-based responses to heat shock. Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways. Many responses were transient, tending to normalize within 24 hours.
In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.