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Open Access Research article

Hin-mediated DNA knotting and recombining promote replicon dysfunction and mutation

Richard W Deibler123, Jennifer K Mann24, De Witt L Sumners4 and Lynn Zechiedrich124*

  • * Corresponding author: Lynn Zechiedrich elz@bcm.edu

  • † Equal contributors

Author Affiliations

1 Interdepartmental Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030-3411 USA

2 Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas 77030-3411 USA

3 Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115 USA

4 Department of Mathematics, Florida State University, Tallahassee, Florida 32306-4510 USA

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BMC Molecular Biology 2007, 8:44  doi:10.1186/1471-2199-8-44

Published: 25 May 2007

Abstract

Background

The genetic code imposes a dilemma for cells. The DNA must be long enough to encode for the complexity of an organism, yet thin and flexible enough to fit within the cell. The combination of these properties greatly favors DNA collisions, which can knot and drive recombination of the DNA. Despite the well-accepted propensity of cellular DNA to collide and react with itself, it has not been established what the physiological consequences are.

Results

Here we analyze the effects of recombined and knotted plasmids in E. coli using the Hin site-specific recombination system. We show that Hin-mediated DNA knotting and recombination (i) promote replicon loss by blocking DNA replication; (ii) block gene transcription; and (iii) cause genetic rearrangements at a rate three to four orders of magnitude higher than the rate for an unknotted, unrecombined plasmid.

Conclusion

These results show that DNA reactivity leading to recombined and knotted DNA is potentially toxic and may help drive genetic evolution.