Figure 4.

Independent ChIP assay demonstrates binding of p63 to ChIPed DNA sequences. ChIP was performed on HaCaT cells with N-terminal RR-14 and C-terminal H-129 anti-p63 antibodies. (A) Enrichment of target genes relative to IgG on an agarose gel. (B) Real-time PCR demonstrating the relative enrichment of p63 target genes with p63 antibodies and IgG. Primer sequences utilized are listed in Additional File 3.