1% agarose gel electrophoresis of the PCR reaction products, carried out using a couple of specific primers (whose sequences are reported in Table 1) flanking the mini-intron region of DAG1a and its counterpart region in DAG1b from T. nigroviridis. The cartoon on the left refers to the different reaction products obtained with appropriate primers (depicted as arrows). The mini-intron is evidenced by an asterisks. The two DNA fragments obtained using respectively genomic or cDNA as a template, differ for the presence of the mini-intron sequence, demonstrating the splicing-out of the mini-intron sequence in T. nigroviridis.
Pavoni et al. BMC Molecular Biology 2007 8:34 doi:10.1186/1471-2199-8-34