Table 2

Frequencies of G418^r colony formation in pDRneo-transfected cells

Cells

Frequency of G418^r

Fold Induction

+ tet

- tet

^a 10IN-SCE.1flp1/DRneo, Clone 1

4.7 × 10^-6

7.2 × 10^-2

1.5 × 10⁴

^a 10IN-SCE.1flp1/DRneo, Clone 2

1.3 × 10^-6

2.9 × 10^-2

2.2 × 10⁴

^a 10IN-SCE.1flp1/DRneo, Clone 3

1.0 × 10^-6

3.5 × 10^-2

3.5 × 10⁴

^b Rht14/DRneo

1.3 × 10^-5

1.4 × 10^-5

1.1

^a Three independent pDRneo-transfected 10IN-SCE.1flp1 clones were grown continuously with tet in the medium (+tet) or tet was withdrawn for 48 h. (-tet) prior to G418 selection (methods).

^b A single hygro^r clone, isolated after the transfection of Rht14 cells with pDRneo, was grown for 48 h. and then selected in G418, with tet either present (+tet) or not present (-tet) in the medium throughout (methods).

Brough et al. BMC Molecular Biology 2007 8:30   doi:10.1186/1471-2199-8-30