Figure 5.

Confirmation of microarray data using qPCR. Relative expression levels of AMACR (A-C) and hepsin (D-F) in benign prostatic epithelial, primary prostate cancer, and metastatic prostate cancer cells laser capture microdissected from 25 cases were measured using qPCR (A, B, D, E) and HG-U133 Plus 2.0 arrays (C and F). Quantitative PCR was performed using cDNA synthesized from RNA samples before (A and D) and after (B and E) linear amplification. For microarray analysis, RNA samples were linearly amplified and labeled using the IVT kit.