Figure 5.

Identification of the minimal bidirectional promoter region of the PDCD10-SERPINI1 gene pair. The 5'- and 3'-deleted putative promoter fragments were individually cloned into the upstream of the firefly luciferase gene (LUC) in the pGL3-Basic vector followed by transient transfection into human neuroglioma H4 cells. The pRL-TK plasmid carrying Renilla luciferase gene was co-transfected as the internal control. The relative firefly luciferase activities were normalized to the Renilla luciferase activities to correct the transfection efficiency. The length and the position of each promoter insert are shown in scale. The numbers denote the positions of nucleotides with respect to +1, the first intergenic nucleotide next to the transcriptional start site of PDCD10, and +851, the intergenic nucleotide right next to the transcriptional start site of SERPINI1. The empty boxes represent inserted intergenic sequences. The shaded boxes represent the SV40 promoter.