|
Relative mRNA levels of C-28/I2 chondrocytes under the influence of IL-1β and curcumin. |
||||||
| Experimental condition |
mRNA relative to untreated control group (mean ± SD) |
|||||
|
|
||||||
| ACTB |
GAPDH |
B2M |
HPRT1 |
SDHA |
YWHAZ |
|
|
|
||||||
| IL-1β |
0.93 ± 0.16 |
0.86 ± 0.10 |
1.06 ± 0.17 |
0.97 ± 0.15 |
0.91 ± 0.11 |
0.92 ± 0.13 |
| Simultaneous incubation with curcumin [c]1 |
0.72 ± 0.07 |
0.74 ± 0.11 |
0.85 ± 0.10 |
0.88 ± 0.11 |
0.76 ± 0.10 |
0.78 ± 0.08 |
| Simultaneous incubation with curcumin [c]2 |
0.41 ± 0.04* |
0.89 ± 0.16 |
0.64 ± 0.06 |
0.86 ± 0.08 |
0.61 ± 0.06 |
0.85 ± 0.11 |
| Pre-incubation with curcumin [c]1 |
0.87 ± 0.13 |
0.98 ± 0.15 |
0.94 ± 0.18 |
0.97 ± 0.11 |
0.95 ± 0.23 |
1.02 ± 0.21 |
| Pre-incubation with curcumin [c]2 |
0.37 ± 0.06* |
0.46 ± 0.05* |
0.28 ± 0.04* |
0.38 ± 0.04* |
0.09 ± 0.02* |
0.65 ± 0.06 |
| Curcumin only |
0.28 ± 0.01* |
0.48 ± 0.06* |
0.31 ± 0.03* |
0.38 ± 0.05* |
0.10 ± 0.01* |
0.63 ± 0.04 |
|
Boldfaced values p < 0.05 * p < 0.05 and change > 2-fold mRNA levels under the treatment with curcumin in different experimental conditions are presented relative to the untreated control group calculated by the equation E-ΔCt (Mean ± SD, n = 9; value of 1 represents average level of mRNA expression in untreated control group). [c]1, low concentration of curcumin (5 μM); [c]2, high concentration of curcumin (50 μM). | ||||||
Toegel et al. BMC Molecular Biology 2007 8:13 doi:10.1186/1471-2199-8-13 |
||||||