Table 7

Distinctions among the three column purification-based protocols used in this study.

Parameter

Protocol 1

Protocol 2

Protocol 3

Histological sections

4 sections of 20 μm (80 μm)

1 section of 10 μm (10 μm)

2 sections of 10 μm (20 μm)

Deparaffinization

1 ml xylene 100% (3 min – 55°C)

800 μl d-limonene (5 min – room temperature)

1 ml d-limonene (10 min – room temperature)

Proteinase K incubation

3 hours – 50°C

"Overnight" – 55°C

3 hours – 55°C

RNA isolation

Solution provide by kit 1

Solution provide by kit 2

0.875 μl mercaptoethanol in 125 μl buffer provide by kit 3

DNase incubation

30 min – 37°C

45 min – 37°C

15 min – 37°C

RNA purification

Solutions provided by the kit

Solutions provided by the kit

Solutions provided by the kit

Ribeiro-Silva et al. BMC Molecular Biology 2007 8:118   doi:10.1186/1471-2199-8-118