|
Comparison of the four protocols for quantity and quality of extracted RNA for only samples that produced biologically-useful RNA (RIN ≥ 1.4) in recently archived months old FFPE samples. Case success is defined for each protocol as the percentage of samples where at least one of three RNA extraction attempts provided a RIN ≥ 1.4. |
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| Months old samples: |
Protocol 1 |
Protocol 2 |
Protocol 3 |
Protocol 4 |
|
|
|
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| FFPE tissue input |
80 μm |
10 μm |
20 μm |
50 μm |
|
| Successful extractions |
34/42 (81%) |
34/42 (81%) |
39/42 (93%) |
35/42 (83%) |
|
| Case success |
14/14 (100%) |
14/14 (100%) |
14/14 (100%) |
14/14 (100%) |
|
| RNA (pg/μl) |
Minimum value |
76 |
152 |
987 |
34 |
| Maximum value |
185,703 |
259,012 |
115,194 |
2,209,739 |
|
| Mean |
24,818 |
64,317 |
26,843 |
180,584 |
|
| Median |
14,781 |
48,255 |
15,146 |
51,245 |
|
| Minimum RIN of 1.4 |
Minimum value |
1.5 |
1.6 |
1.4 |
1.4 |
| Maximum value |
5.1 |
2.7 |
2.6 |
4.9 |
|
| Mean |
2.3 |
2.1 |
2.3 |
2.3 |
|
| Median |
2.3 |
2.0 |
2.4 |
2.2 |
|
Ribeiro-Silva et al. BMC Molecular Biology 2007 8:118 doi:10.1186/1471-2199-8-118 |
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