|
Comparison of the four protocols for quantity and quality of extracted RNA for only samples that produced biologically-useful RNA (RIN ≥ 1.4) in ten year old FFPE samples. Case success is defined for each protocol as the percentage of samples where at least one of three RNA extraction attempts provided a RIN ≥ 1.4. |
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| Ten year old samples: |
Protocol 1 |
Protocol 2 |
Protocol 3 |
Protocol 4 |
|
|
|
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| FFPE tissue input |
80 μm |
10 μm |
20 μm |
50 μm |
|
| Successful extractions |
26/42 (62%) |
26/42 (62%) |
35/42 (83%) |
30/42 (71%) |
|
| Case success |
12/14 (86%) |
14/14 (100%) |
13/14 (93%) |
13/14 (93%) |
|
| RNA (pg/μl) |
Minimum value |
1,236 |
1,650 |
1,314 |
9,864 |
| Maximum value |
73,861 |
165,588 |
137,594 |
314,693 |
|
| Mean |
17,754 |
51,985 |
30,160 |
107,042 |
|
| Median |
13,692 |
31,104 |
20,229 |
86,682 |
|
| Minimum RIN of 1.4 |
Minimum value |
1.4 |
1.5 |
1.9 |
1.8 |
| Maximum value |
2.5 |
5.2 |
4.4 |
6.1 |
|
| Mean |
2.0 |
2.5 |
2.3 |
2.4 |
|
| Median |
2.1 |
2.3 |
2.3 |
2.2 |
|
Ribeiro-Silva et al. BMC Molecular Biology 2007 8:118 doi:10.1186/1471-2199-8-118 |
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