Table 4

Overall comparison of the four RNA extraction protocols for quantity (in pg/μl) and quality (RIN) for recently archived months old FFPE samples.

Months old samples:

Protocol 1

Protocol 2

Protocol 3

Protocol 4


FFPE tissue input

80 μm

10 μm

20 μm

50 μm

Successful extractions

36/42 (86%)

34/42 (81%)

42/42 (100%)

35/42 (83 %)

RNA (pg/μl)

Minimum value

76

152

672

34

Maximum value

185,703

259,012

115,194

2,209,739

Mean

25,133

64,317

26,808

180,584

Median

14,782

48,255

14,599

51,245

RIN

Minimum value

1.1

1.6

1.0

1.4

Maximum value

5.1

2.7

2.6

4.9

Mean

2.2

2.1

2.2

2.3

Median

2.3

2.0

2.4

2.2


Ribeiro-Silva et al. BMC Molecular Biology 2007 8:118   doi:10.1186/1471-2199-8-118

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