Additional file 3.

The WT TIF-IA protein is synthesized in cells with and without the exonization. Total protein was extracted from the indicated cells and analyzed by Western blotting using polyclonal rabbit anti-TIF-IA serum (kindly gifted from the Grummt's lab) at a 1/10,000 dilution. 293T and U2OS are human cell lines, and 3T3 is a murine cell line.

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Amit et al. BMC Molecular Biology 2007 8:109   doi:10.1186/1471-2199-8-109