Table 1

ΔG of SD:aSD interactions.

Shine-Dalgarno

ΔG37°C

ΔG20°C

(kcal/mol)

(kcal/mol)


    UAAGGAGG

-9.4

-12.6

A

    AAGGAGG

-9.3

-12.3

AU

    AGGAGG

-7.7

-10.1

AUU

    GGAGG

-6.9

-9.4

AUUC

    GAGG

-3.9

-5.7

AUU

    GGAG
C

-4.7

-6.7

AUUCC

    AGG

-1.0

-2.1

AUUC

    GAG
C

-1.7

-2.9

AUUCCU

    GG

-0.1

-1.3

AUUCCUC

    G

NA

NA


ΔG of SD:aSD interactions at 37°C and 20°C, including 8 nucleotides from mRNA and 8 nucleotides from 16S rRNA. Free energy was calculated using hybrid-min from the UNAFold package [45].

Vimberg et al. BMC Molecular Biology 2007 8:100   doi:10.1186/1471-2199-8-100

Open Data