Intracellular co-location of PDE9A1, PDE9A2 and PDE9A3. Intracellular co-location of PDE9A1-, PDE9A2- and PDE9A3-GFP chimeric proteins with endoplasmic reticulum, Golgi apparatus and membrane markers in transiently transfected Cos-1 and HeLa cells. Confocal analysis of PDE9A1-GFP and PDE9A3-GFP with (A) calnexin (endoplasmic reticulum marker) and (B) giantin (Golgi apparatus marker). (C) Membrane location of PDE9A1- and PDE9A2-GFP chimeric proteins in transiently transfected HeLa cells by in vivo fluorescence microscopy. Black and white panels show the expression of GFP-carboxy terminal-fused proteins and the marker (calnexin, giantin and FM4-64) location, and the coloured image show the merged composition of both images. White arrows indicate the co-location of the chimeric proteins with the subcellular markers.
Rentero and Puigdomènech BMC Molecular Biology 2006 7:39 doi:10.1186/1471-2199-7-39