Intracellular localization of GFP-chimeric PDE9A splice variants in transiently transfected HeLa cells. All splice variant constructions were cloned with their 5'UTR region as described in Methods. Confocal analysis of: a and b, GFP-carboxy terminal-fused proteins of PDE9A1; c, N-terminal region of PDE9A1; d, C-terminal region of PDE9A; e, PDE9A2; f, PDE9A3; g, PDE9A17; and h, GFP control. Panel b is a detailed image of GFP-PDE9A1. White arrows indicate the membrane location of chimeric proteins.
Rentero and Puigdomènech BMC Molecular Biology 2006 7:39 doi:10.1186/1471-2199-7-39