Differential start codon usage in PDE9A splice variants. (A) Western blot analysis of the expression of the chimeric PDE9A splice variants fused to GFP. GFP-fused proteins of PDE9A1 (lane 1, 96 kDa), the N-terminal region of PDE9A1 (lane 2, 62 kDa), PDE9A2 (lane 3, 89 kDa), PDE9A3 (lane 4, 81 kDa), and PDE9A17 (lane 5, 91 kDa) were produced in transfected Cos-1 cells, and were immunoblotted using a rabbit anti-GFP serum (1:1000). (B) Sequence comparison between Kozak consensus motif and PDE9A start codons, where the critical -3 and +4 positions are marked. (C) Schematic secondary structure representation of PDE9A exon 1 sequence. Start codons are emphasized by grey boxes.
Rentero and Puigdomènech BMC Molecular Biology 2006 7:39 doi:10.1186/1471-2199-7-39