DNA contamination in total RNA isolated from Saccharomyces cerevisiae. Total RNA was isolated from Saccharomyces cerevisiae cells harvested at cellular density of 1.2 mg (dry weight/mL). PCR and RT-PCR amplicons were generated by 30 reactions cycles using ACT1 or CNA1 primers and 1.0 μg of RNA. Ten micro liters of each reaction was electrophoresed on a 1.3% agarose gel and stained with ethidium bromide. A – ACT1 amplicons. B – CNA1 amplicons. Untreated total RNA amplified by PCR (lane 1) or RT-PCR (lane 2); treated samples according to protocol I, followed by PCR (lane 3) or RT-PCR amplifications (lane 4); treated samples according to protocol II followed by PCR (lane 5) or RT-PCR amplification (lane 6).
Del Aguila et al. BMC Molecular Biology 2005 6:9 doi:10.1186/1471-2199-6-9