Table 1 |
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|
Overview of all tested interactions within the Dnmt family. |
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|
cloned in pACT2 |
cloned in pBMT117c.1 |
lacZ |
HIS3 |
Comments |
|
|
|
|||||
|
Dnmt 2 |
N-Dnmt 1 |
- |
- |
||
|
N-Dnmt 3a |
C-Dnmt 1 |
- |
- |
||
|
C-Dnmt 3a |
N-Dnmt 1 |
- |
- |
||
|
Dnmt 1 |
N-Dnmt 3b |
C-Dnmt 1 |
- |
- |
|
|
C-Dnmt 3b |
N-Dnmt 1 |
- |
- |
||
|
vs |
DNMT 3L |
C-Dnmt 1 |
- |
- |
|
|
DNMT 3L |
N-Dnmt 1 |
(+/+) |
- |
weak autoactivation |
|
|
DNMT 3L |
control |
(+/+) |
- |
weak autoactivation |
|
|
other Dnmt's |
N-Dnmt 1 |
DNMT 3L |
- |
- |
|
|
N-Dnmt 1s |
DNMT 3L |
- |
- |
short isoform Dnmt1 |
|
|
DNMT 3L |
N-Dnmt 1s |
(+) |
- |
weak autoactivation |
|
|
Dnmt 2 |
C-Dnmt 1 |
- |
- |
||
|
|
|||||
|
Dnmt 3a |
control |
C-Dnmt 3a |
- |
- |
|
|
control |
C-Dnmt 3b |
- |
- |
||
|
vs |
C-Dnmt 3a |
N-Dnmt 3a |
- |
- |
|
|
C-Dnmt 3b |
N-Dnmt 3b |
- |
- |
||
|
Dnmt 3b |
N-Dnmt 3a |
C-Dnmt 3a |
- |
- |
|
|
N-Dnmt 3b |
C-Dnmt 3b |
- |
- |
||
|
|
|||||
|
DNMT 3L |
control |
(+/+) |
- |
weak autoactivation |
|
|
control |
C-Dnmt 3a |
- |
- |
||
|
DNMT 3L |
control |
C-Dnmt 3b |
- |
- |
|
|
DNMT 3L |
N-Dnmt 3a |
(+) |
- |
weak autoactivation |
|
|
vs |
DNMT 3L |
N-Dnmt 3b |
(+) |
- |
weak autoactivation |
|
DNMT 3L |
C-Dnmt 3a +++/+++ |
++/++ |
|||
|
other Dnmt's |
C-Dnmt 3a |
DNMT 3L |
+++ |
+++ |
|
|
DNMT 3L |
C-Dnmt 3b |
+/+ |
- |
||
|
C-Dnmt 3b |
DNMT 3L |
+++ |
+++ |
||
|
Dnmt 2 |
DNMT 3L |
- |
- |
||
|
|
|||||
|
Dnmt 2 |
Dnmt 2 |
control |
- |
- |
|
|
Dnmt 2 |
N-Dnmt 1 |
- |
- |
||
|
vs |
Dnmt 2 |
C-Dnmt 1 |
- |
- |
|
|
Dnmt 2 |
N-Dnmt 3a |
- |
- |
||
|
other Dnmt's |
Dnmt 2 |
N-Dnmt 3b |
- |
- |
|
|
Dnmt 2 |
DNMT 3L |
- |
- |
||
|
|
|||||
|
MBD 2 |
MBD 3 |
++ |
++ |
positive control |
|
|
MBD 2 |
N-Dnmt 1 |
- |
- |
||
|
MBD2 |
MBD 2 |
C-Dnmt 1 |
- |
- |
|
|
MBD 2 |
N-Dnmt 3a |
- |
- |
||
|
vs |
MBD 2 |
N-Dnmt 3b |
- |
- |
|
|
MBD 2 |
DNMT 3L |
- |
- |
||
|
Dnmt's |
Dnmt 2 |
MBD 2 |
++ |
+++ |
strong autoactivation |
|
Dnmt 2 |
MBD 2 |
++ |
+++ |
strong autoactivation |
|
|
control |
MBD 2 |
++ |
+++ |
strong autoactivation |
|
|
|
|||||
|
MBD3 |
MBD 3 |
N-Dnmt 1 |
- |
- |
|
|
MBD 3 |
C-Dnmt 1 |
- |
- |
||
|
vs |
MBD 3 |
N-Dnmt 3a |
- |
- |
|
|
MBD 3 |
N-Dnmt 3b |
- |
- |
||
|
Dnmt's |
MBD 3 |
DNMT 3L |
- |
- |
|
|
|
|||||
|
The results are grouped in six panels corresponding to the different proteins tested. To allow for completeness within each panel, some of the results are presented in two different panels. N-Dnmt1s corresponds to the N-terminal domain of the short isoform, while the other abbreviations are explained in the text. Duplicate results are separated by a (/). |
|||||
|
Margot et al. BMC Molecular Biology 2003 4:7 doi:10.1186/1471-2199-4-7 |
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