Comparison of the chimeric c-myc antisense 20-mer, 5'F-MD757AS with its CpG motif as an isolated 5-mer oligodeoxynucleotide for effects on c-Myc protein levels and induction of apoptosis in MOLT-4 cells and a MOLT-4 CpG 5-mer-resistant subline. (A) The antisense 20-mer reduced intracellular concentrations of c-Myc protein in MOLT-4 cells more effectively than the 5-mer. (B) Both oligodeoxynucleotides induced apoptosis in parental MOLT-4 cells. (C) The antisense 20-mer also reduced the level of c-Myc protein in the MOLT-4 subline selected for resistance to CGTTA whereas the 5-mer had no significant effect. (D) Neither oligodeoxynucleotide induced apoptosis or cell cycle arrest in the CpG 5-mer-resistant subline. Viable cell number was determined by flow cytometric counting of cells excluding propidium iodide. Values in all panels are the mean and standard deviation of 3 replicates.
Tidd et al. BMC Molecular Biology 2001 2:13 doi:10.1186/1471-2199-2-13