KLF3 binds the human and mouse α-globin promoters in vivo in chromatin immunoprecipitation assays. An anti-KLF3 antibody was used to immunoprecipitate chromatin from the following cell types: (A) uninduced MEL cells, (B) induced MEL cells, (C) mouse primary erythroblasts, (D) uninduced interspecific MEL hybrids containing a normal copy of human chromosome 16, (E) induced interspecific MEL hybrids, and (F) human primary erythroblasts. The y-axis represents enrichment over input DNA, normalized to a control sequence in the Gapdh gene (mouse) or 18S (human). The x-axis represents the positions of the TaqMan probes used. The coding sequence is represented by the three exons (Promoter/Ex1, Ex2, and Ex3) of the α-globin genes. HS- primer sets refer to upstream DNase-hypersensitive regions. Zeta pr refers to the mouse and human embryonic α-globin promoters (Hba-x and HBZ). Inter, refers to the intergenic region (between mouse Hba-a1 and Hba-a2). 5' and 3' are negative controls flanking the α-globin gene. β-actin and β-globin denote control sequences at the β-actin gene and β-globin promoter respectively. Error bars correspond to ±1 standard deviation from at least two independent ChIPs.
Funnell et al. BMC Molecular Biology 2014 15:8 doi:10.1186/1471-2199-15-8