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Open Access Highly Accessed Research article

Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter

Minji Liu1*, Xiaoyan Wang2, Ya Peng3, Shourong Shen25* and Guiyuan Li4

Author Affiliations

1 Department of Gastroenterology, Zhuzhou central Hospital, 412007 Zhuzhou, Hunan, China

2 Department of Gastroenterology, Third Affiliated Hospital of Xiangya School of Medicine, Central South University, 410013 Changsha, Hunan, China

3 Department of Digestive Diseases, Hunan Provincial People’s Hospital, 410005 Changsha, Hunan, China

4 Cancer Research Institute, Central South University, 410078 Changsha, Hunan, China

5 Hunan Key laboratory of Nonresolving Inflammation and Cancer, 410013 Changsha, Hunan, China

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BMC Molecular Biology 2014, 15:14  doi:10.1186/1471-2199-15-14

Published: 16 July 2014

Abstract

Background

As a novel candidate metastasis suppressor gene, Nasopharyngeal carcinoma-associated gene 6 (NGX6) is involved in cellular growth, cell cycle progression and tumor angiogenesis. Previous studies have shown that NGX6 gene is down-regulated in colorectal cancer (CRC). However, little is known about its transcriptional regulation.

Results

We defined the minimal promoter of NGX6 gene in a 186-bp region (from-86 to +100) through mutation construct methods and luciferase assays. Results from Electrophoretic mobility shift assays (EMSA) and Chromatin immunoprecipitation (ChIP) revealed that Early growth response gene 1 (Egr-1) binds to the Sp1/Egr-1 overlapping site of NGX6 minimal promoter. Overexpression of Egr-1 increased the promoter activity and mRNA level of NGX6 gene; while knock-down of endogenous Egr-1 decreased mRNA expression of NGX6 gene.

Conclusion

These results demonstrate that Egr-1 regulates NGX6 gene transcription through an overlapping Sp1/Egr-1 binding site as a positive regulator of NGX6 gene.